Drosophila Genome Editing Services

Fly Genome Editing Expert — WellGenetics

WellGenetics is dedicated to providing researchers professional services in generating transgenes and gene knockout/knockin in fly models (Drosophila melanogaster). We are an independent company from any academic research institutes. Each project will be designed, executed, and supervised by postdoctoral scientists, thus to ensure the quality and reliability of entire experiment process.

Simply let us know the name of gene and how you want it to be edited. We will propose the best strategy and do the rest of steps for you — from Design to Fly! Please see service lists below.

Free Consulting Service: Strategy & Plan

Below are 8 most popular genome editing requests: Gene Disruption, Gene Knockout, Reporter Knockin, Tag Knockin, Point Mutation/Seamless Editing, Variant Replacement, Conditional Knockout/Knockin Editing, and Using Existing Tools. Any special editing request other than listed is welcomed. WellGenetics scientists will suggest strategies to meet specifically to your needs and propose a most practical and economic way to achieve what you want!

Here we list cassette examples for your reference. The alternative selection markers are:

Feel free to ask us your favor combination, and we can check our cassette library.

Gene Disruption Service Packages
A Pre-mature Allele

Knockout Service Packages
B Gene knockout

Reporter Knockin Service Packages
C1 Reporter replacement with exogenous Kozak
C2 Reporter replacement with endogenous ATG
C3 N-terminal Reporter Knock-in and Gene Disruption
C4 C-terminal Reporter Knock-in
C5 Gene Knockout and Reporter Knock-in

Tag Knockin Service Packages
D1 N-terminal Tagging with LoxP System (a peptide linker with 46a.a. between tag and gene)
D2 C-terminal Tagging with LoxP System (a sequence linker between tag and 3'UTR)
D3 N-terminal Tagging with PBac System (a peptide linker with 2 a.a. between tag and gene or seamless)
D4 C-terminal Tagging with PBac System (a sequence linker between tag and 3'UTR or seamless)
D5 Internal Tagging with PBac System (a peptide linker with 2 a.a. between tag and gene or seamless)

Point mutation/Seamless Service Packages
E1 Point mutation/Seamless Service Packages	E2 Domain Deletion

Variant Replacement Service Packages
F1 Gene Replacement (Humanized Fly)
F2 Variant Rescue: by CRISPR Re-Targeting
F3 Variant Rescue: by PhiC31 Site-Specific Recombination
F4 Variant Rescue: by RMCE swap

Conditional Knockout/Knockin Service Packages
G1 Conditional Knockout	G2 Conditional Tagging

Using Existing Tools
H1 T-GEM/CRIMIC knockin using available cassette and RMCE swap
H2 RMCE swap using existing fly tools
H3 PhiC31 Site-Specific Recombination using existing fly tools

CRISPR Service Package with Selection Marker -or- with PCR Screening

WellGenetics provides CRISPR Service Package including Design, Cloning, Microinjection, Screening and Stock Establishment to bring you gene-edited flies.

Simply let us know the name of gene and how you want it to be edited. We propose the best strategy and do the rest of steps for you - from Design to Fly.

Using homology-dependent repair (CRISPR/HDR), we can knockin desired cassette in combination with cuts by 1 or 2 guide RNAs.

We welcome any project that requires customized designs to fit your specific research needs. Feel free to show us your interests in CRISPR fly!

Add-on Services

Want to Know More about Our Services?

Send your request to us, we will contact you shortly!
Or read our Demo Case Report.

 Send Your Request　　Demo Case Report