Drosophila CRISPR Genome Editing Services

Fly Genome Editing Expert — WellGenetics

WellGenetics is dedicated to providing researchers professional services in generating transgenes and gene knockout/knockin in fly models (Drosophila melanogaster). We are an independent company from any academic research institutes. Each project will be designed, executed, and supervised by postdoctoral scientists, thus to ensure the quality and reliability of entire experiment process.

Simply let us know the name of gene and how you want it to be edited. We will propose the best strategy and do the rest of steps for you — from Design to Fly! Please see service lists below.


Free Consulting Service: Strategy & Plan

Below are 4 most popular genome editing requests: Gene Disruption, Reporter Knock-in, Tag Knock-in, and Seamless Editing. Any special editing request other than listed is welcomed. WellGenetics scientists will suggest strategies to meet specifically to your needs and propose a most practical and economic way to achieve what you want!

Gene Disruption

Selection Marker Options

Reporter Knock-in

Tag Knock-in

Seamless Editing


CRISPR Service Package with
Selection Marker -or- with PCR Screening

WellGenetics provides CRISPR Service Package including Design, Cloning, Microinjection, Screening and Stock Establishment to bring you gene-edited flies.

Simply let us know the name of gene and how you want it to be edited. We propose the best strategy and do the rest of steps for you - from Design to Fly.

Using homology-dependent repair (CRISPR/HDR), we can knockin desired cassette in combination with cuts by 1 or 2 guide RNAs.

We welcome any project that requires customized designs to fit your specific research needs. Feel free to show us your interests in CRISPR fly!

 


Add-on Services

Customized Cassette Cloning Service
Design and construct cassette for your specific research needs.

gRNA Transgenic Fly Generation Service
Microinject gRNA plasmid into requested landing site and set up the gRNA transgenic stock.

Gene Synthesis Service
To avoid confusion of polymor-phism or PCR errors occurred in PCR cloning, you may choose to synthesize homology arms.

Selection Marker Excision Service
To excise selection marker floxed by loxP/PBac, we provide cre/loxP excision and PBac transposition services.

 

PCR Screening Service
Individual F1 progeny is back-crossed to balancer. We then perform single-fly genomic PCR on each fertile F1 flies.

HRMA Screening
Individual F1 progeny is back-crossed to balancer for high resolution melting analysis (HRMA). Using HRMA to screen for single-nucleotide mismatches or indel mutations.

Digestion Screening
Individual F1 progeny is back-crossed to balancer. We then perform single-fly genomic PCR on each fertile F1 flies. PCR products will be purified and cut by endonuclease and analyzed by gel eletrophoresis.

 

Want to Know More about Our Services?

Send your request to us, we will contact you shortly!
Or read our Demo Case Report.

   Demo Case Report