PhiC31 Microinjection & Screening Services

• 200-embryos microinjection, G0 cross & F1 screen (w+). We return unbalanced positive lines.
• We have VK33, VK37, attP2, attP40, ZH51C & ZH86Fb as regular panel of landing sites. You can also choose any other sites listed in Bloomington Stock Center and allow two generations of amplification time.
• We guarantee 1-3 transformants for plasmid at standard size (<13kb).
• No guarantee for large plasmid (>13kb).

P-element Microinjection & Screening Services

• 200-embryos microinjection, G0 cross & F1 screen (w+). We return unbalanced positive lines.
• We guarantee 5-8 transformants for plasmid at standard size (<13kb).
• No guarantee for large plasmid (>13kb).

Bac (P[acman]) Microinjection & Screening Services

• 600-embryos microinjection, G0 cross & F1 screen (w+). We return unbalanced positive lines.
• No guarantee for large plasmid (>13kb).

CRISPR Microinjection

• 200-embryos microinjection. We return survived larvae after microinjection.

Non-melanogaster Microinjection

• 200-embryos microinjection. We return survived pupae after microinjection.

Mosquito Microinjection

• 500-embryos microinjection. We return injected embryos after microinjection.

Balancing Service
Isogenize & balance positive lines from screening. We establish fly stocks for you.

Plasmid Construction Service (<3kb)
Maximum 3 PCR-fragments and together length below 3kb. We use High-Fidelity Phusion DNA Polymerase to amplify the fragments and sequence through all parts we cloned in. We return bacteria streaking plate and 4µg plasmid DNA.

Plasmid DNA Prep.
Plasmid preperation to provide high quality DNA for microinjection. Validation service by restriction enzyme digestion or PCR is included!

BAC DNA Prep.
Qiagen Large Construct Kit for BAC DNA preperation (>20kb) to provide high quality DNA for microinjection. Validation service by restriction enzyme digestion or PCR is included!.